颱風動態

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Thisliquidmediumwasdispensedin30mllotsinto250mlbottlesandsterilizedintheautoclaveat1.02kgcm_2pressureandtemperatureof121℃for15min.Aftercooling,eachbottlewasinoculatedwitha7mm(diameter)myceliamatof5-day-oldactively-growingcultureofP.atroumbonataand...顯示更多Thisliquidmediumwasdispensedin30mllotsinto250mlbottlesandsterilizedintheautoclaveat1.02kgcm_2pressureandtemperatureof121℃for15min.Aftercooling,eachbottlewasinoculatedwitha7mm(diameter)myceliamatof5-day-oldactively-growingcultureofP.atroumbonataandincubatedat10,15,20,25,30,35,40and45℃for7days.Eachtreatmentwasthricereplicated.ThemyceliawereharvestedusingapreweighedWhatmanfilterpaperandoven-driedat80℃untilconstantweightwasobtained(Chandra&Purkayastha,1977).



把每30ml的這個液態培養基放在一個250ml的瓶子中, 用壓力1.02kg/平方公分,溫度121度C的壓力鍋中消毒15分鐘. 等它們冷卻後, 每個瓶子接芽直徑7mm 5天大的菌絲叢, .....在10, 15, 20, 25, 30, 35, 40 and 45℃的條件下培養7天, 每個條件都重複3次, ... ~>_


翻：這一種液體媒體在1.02公斤cm_2壓力和121℃的溫度被在快鍋在30毫升運氣進入250毫升瓶子之內分發而且使成不毛15分鐘。在冷卻之後，每支瓶子與7公釐（直徑）mycelia墊一起接種5天的－舊的積極地＆ndash；P的增加文化。atroumbonata而且在10,15,20,25,30,35,40和45℃抱7天。每治療三倍三次被複製。mycelia被收割使用被秤重華特曼紙過濾器紙和烤箱＆ndash的pre；弄乾在80℃unt。

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